シークエンサー

• 従来のシークエンサー
  • Maxam-Gilbert method（化学分解法）
  • Sanger method（酵素法）
• 次世代シークエンサー
  • 第二世代
    • 2base encoding (with emulsion PCR): 5500 SOLiD (Thermo)
    • pH alteration (with emulsion PCR): Ion torrent (Thermo)
    • pyro-sequence (with emulsion PCR): GS junior, GS FLX Titanium XL+ (Roche)
    • step by step, fluorescence detection (with Bridge PCR): Genome Analyzer IIx, HiSeq2500, MiSeq, NextSeq 500 (illumina)
  • 第三〜四世代
    • Single molecular real-time sequencing: PacBio RS, PacBio SEQUEL (Pac Bio)
    • Single molecular real-time sequencing/Current alteration: MinION, GridION, PromethION, SmadgION (ONT)